ABHD14B, 1-210aa, Human, His tag, E Coli

Instruction Manual!

Product name:	ABHD14B, 1-210aa, Human, His tag, E Coli
Source:	Human
Purity:	> 95%
Buffer Formulation:	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Applications:	SDS-PAGE
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name ABHD14B, Recombinant Protein
Also Known As ABHD14B, 1-210aa, Human, His tag, E Coli
Product Synonym Names Abhydrolase domain containing 14B; CIB
Product Gene Name ABHD14B recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    MGSSHHHHHH SSGLVPRGSH MGSHMAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSE TWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMY SLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGA GHPCYLDKPE EWHTGLLDFL QGLQ

Purity/Purification    > 95% by SDS - PAGE

Form/Format    Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl

Concentration    0.5mg/ml (determined by Bradford assay) (lot specific)

Antigen Species    Human

Tag    His-tag

Expression System    E Coli

Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

Other Notes    Small volumes of ABHD14B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ABHD14B recombinant protein
   ABHD14B (abhydrolase domain containing 14B) belongs to the AB hydrolase superfamily. This protein contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro). It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family forABHD14B recombinant protein    Enzymes & Proteases

Applications Tested/Suitable for ABHD14B recombinant protein
SDS-PAGE

SDS-PAGE of ABHD14B recombinant protein

Sequence		MGSSHHHHHH SSGLVPRGSH MGSHMAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSE TWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMY SLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGA GHPCYLDKPE EWHTGLLDFL QGLQ


Purity/Purification		> 95% by SDS - PAGE
Form/Format		Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100mM NaCl
Concentration		0.5mg/ml (determined by Bradford assay) (lot specific)

Antigen Species		Human
Tag		His-tag
Expression System		E Coli

Preparation and Storage		Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
Other Notes		Small volumes of ABHD14B recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ABHD14B recombinant protein		ABHD14B (abhydrolase domain containing 14B) belongs to the AB hydrolase superfamily. This protein contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro). It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Product Categories/Family forABHD14B recombinant protein		Enzymes & Proteases

Applications Tested/Suitable for ABHD14B recombinant protein		SDS-PAGE

SDS-PAGE of ABHD14B recombinant protein