Recombinant A Kinase Anchor Protein 12 (AKAP12)

Instruction Manual!

Product name:	Recombinant A Kinase Anchor Protein 12 (AKAP12)
Source:	Prokaryotic expression
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Kinase Anchor Protein 12 (AKAP12), Recombinant Protein
Also Known As Recombinant A Kinase Anchor Protein 12 (AKAP12)
Product Gene Name AKAP12 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- PVIVSA TTKKGLSSDL EGEKTTSLKW KSDEVDEQVA CQEVKVSVAI EDLEPENGIL ELETKSSKLV QNIIQTAVDQ FVRTEETATE MLTSELQTQA HVIKADSQDA GQETEKEGEE PQASAQDETP ITSAKEESES TAVGQAHSDI SKDMSEASEK TMTVEVEGST VNDQQLEEVV LPSEEEGGGA GTKSVPEDDG HALLAERIEK SLVEPKEDEK GDDVDDPENQ NSALADTDAS GGLTKESPDT NGPKQKEKED AQEVELQEGK VHSESDKAIT PQAQEELQKQ ERESAKSELT ES

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Host    Host: E Coli
Source: Prokaryotic expression

Purity/Purification    >95%

Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Concentration    4.4 (lot specific)

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Predicted Molecular Mass    36.0kDa

Accurate Molecular Mass (KD)    56kDa

Endotoxin    <1.0EU per 1ug (determined by the LAL method)

Expression System    Prokaryotic expression

Tag    N-terminal His-Tag, T7 tag

Organism Species    Homo sapiens (Human)

Fragment    Pro1485~Ser1782

Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.

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Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of AKAP12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable forAKAP12 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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SDS-Page of AKAP12 recombinant protein    AKAP12 recombinant protein SDS-Page image

Sequence		The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- PVIVSA TTKKGLSSDL EGEKTTSLKW KSDEVDEQVA CQEVKVSVAI EDLEPENGIL ELETKSSKLV QNIIQTAVDQ FVRTEETATE MLTSELQTQA HVIKADSQDA GQETEKEGEE PQASAQDETP ITSAKEESES TAVGQAHSDI SKDMSEASEK TMTVEVEGST VNDQQLEEVV LPSEEEGGGA GTKSVPEDDG HALLAERIEK SLVEPKEDEK GDDVDDPENQ NSALADTDAS GGLTKESPDT NGPKQKEKED AQEVELQEGK VHSESDKAIT PQAQEELQKQ ERESAKSELT ES

Host		Host: E Coli Source: Prokaryotic expression

Purity/Purification		>95%
Form/Format		Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration		4.4 (lot specific)

Predicted Molecular Mass		36.0kDa
Accurate Molecular Mass (KD)		56kDa
Endotoxin		<1.0EU per 1ug (determined by the LAL method)
Expression System		Prokaryotic expression
Tag		N-terminal His-Tag, T7 tag
Organism Species		Homo sapiens (Human)
Fragment		Pro1485~Ser1782
Usage		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of AKAP12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable forAKAP12 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of AKAP12 recombinant protein