Recombinant A Disintegrin And Metalloproteinase With ThrombosPondin 5 (ADAMTS5)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloproteinase With ThrombosPondin 5 (ADAMTS5)
Source:	Prokaryotic expression
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloproteinase With ThrombosPondin 5 (ADAMTS5), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloproteinase With ThrombosPondin 5 (ADAMTS5)
Product Gene Name ADAMTS5 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
RQVE LLLVADSSMA KMYGRGLQHY LLTLASIANR LYSHASIENH IRLAVVKVVV LTDKSLEVSK NAATTLKNFC KWQHQHNQLG DDHEEHYDAA ILFTREDLCG HHSCDTLGMA DVGTICSPER SCAVIEDDGL HAAFTVAHEI GHLLGLSHDD SKFCEENFGS TEDKRLMSSI LTSIDASKPW SKCTSATITE FLDDGHGNCL LDVPRKQILG PEELPGQTYD ATQQCNLTFG PEYSVCPGMD VCARLWCAVV RQGQMVCLTK KLPAVEGTPC GKGRICLQGK CVDKTKKKYY STSSHGNWGS WGPWGQCSRS CGGGVQFAYR HCNNPAPRNS GRYCTGKRAI YRSCSVIPCP PN

OMIM    605007

Host    Host: E Coli
Source: Prokaryotic expression

Purity/Purification    >95%

Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted Molecular Mass    42.9kDa

Accurate Molecular Mass (KD)    43kDa

Endotoxin    <1.0EU per 1ug (determined by the LAL method)

Expression System    Prokaryotic expression

Tag    N-terminal His-Tag

Organism Species    Rattus norvegicus (Rat)

Fragment    Arg267~Asn622

Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAMTS5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAMTS5 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of ADAMTS5 recombinant protein

Sequence		The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. RQVE LLLVADSSMA KMYGRGLQHY LLTLASIANR LYSHASIENH IRLAVVKVVV LTDKSLEVSK NAATTLKNFC KWQHQHNQLG DDHEEHYDAA ILFTREDLCG HHSCDTLGMA DVGTICSPER SCAVIEDDGL HAAFTVAHEI GHLLGLSHDD SKFCEENFGS TEDKRLMSSI LTSIDASKPW SKCTSATITE FLDDGHGNCL LDVPRKQILG PEELPGQTYD ATQQCNLTFG PEYSVCPGMD VCARLWCAVV RQGQMVCLTK KLPAVEGTPC GKGRICLQGK CVDKTKKKYY STSSHGNWGS WGPWGQCSRS CGGGVQFAYR HCNNPAPRNS GRYCTGKRAI YRSCSVIPCP PN
OMIM		605007

Host		Host: E Coli Source: Prokaryotic expression

Purity/Purification		>95%
Form/Format		Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted Molecular Mass		42.9kDa
Accurate Molecular Mass (KD)		43kDa
Endotoxin		<1.0EU per 1ug (determined by the LAL method)
Expression System		Prokaryotic expression
Tag		N-terminal His-Tag
Organism Species		Rattus norvegicus (Rat)
Fragment		Arg267~Asn622
Usage		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAMTS5 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAMTS5 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of ADAMTS5 recombinant protein