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Recombinant Human Forkhead Box Protein O3 (FOXO3)

Recombinant Human Forkhead Box Protein O3 (FOXO3) Recombinant Human Forkhead Box Protein O3 (FOXO3)

Instruction Manual!

Product name: Recombinant Human Forkhead Box Protein O3 (FOXO3)
Source:E. coli
Purity:> 90%
Buffer Formulation:20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
Storage:Avoid repeated freeze/thaw cycles.
UOM:50µg/100µg/200µg/1mg/5mg

[ PROPERTIES ]



 

Figure 2. SDS-PAGE

Source: Prokaryotic expression

Host: E.coli

Residues: Leu369~Met633

Tags: N-terminal His Tag

Subcellular Location: Membrane, Nucleus, Mitochondrion, Cytoplasm

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:

Predicted Molecular Mass: 32.0kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

 

[ SEQUENCE ]


 

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.




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