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NOTCH3) Antibody
Instruction Manual!
| Product name: | NOTCH3) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-NOTCH3) Antibody
Catalog Number：IC105612
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Product Profile
| Product Name | Rabbit anti-NOTCH3) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen |
Synthetic peptide directed towards the C terminal of human NOTCH3 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | NOTCH3 |
|---|---|
| Gene Synonyms |
PCGF3
|
| Gene Full Name | Notch 3 |
| Gene Summary |
NOTCH3 encodes the third discovered human homologue of the Drosophilia melanogaster type I membrane protein notch. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signalling pathway that plays a key role in neural development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remains to be determined. Mutations in NOTCH3 have been identified as the underlying cause of cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
More
|
| Alternative Names |
RNF3
More
DONG1 RNF3A |
| MolecularWeight(MW) | 244kDa |
| Sequence | 2321 amino acids |
Database Links
| Entrez Gene | 4854 |
|---|---|
| SwissProt ID | Q9UM47 |
| Protein Accession | NP_000426 |
Application
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Application
WB Suggested Anti-PCGF3 Antibody Titration: 0.125ug/ml
ELISA Titer: 1:12500
Positive Control: Raji cell lysatePCGF3 is strongly supported by BioGPS gene expression data to be expressed in Human Raji cells
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Application
Host: Rabbit
Target Name: PCGF3
Sample Type: Fetal Muscle lysates
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: F13B
Sample Tissue: Human DLD1
Antibody Dilution: 1.0ug/ml -
Application
Rabbit Anti-F13B antibody
Catalog Number: ARP41999
Formalin Fixed Paraffin Embedded Tissue: Human Fetal Liver
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec -
Application
Rabbit Anti-F13B antibody
Catalog Number: ARP41999
Formalin Fixed Paraffin Embedded Tissue: Human Vessel
Primary antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20x
Exposure Time: 0.5-2.0sec -
Application
WB Suggested Anti-FBXO25 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human heart -
Application
Host: Rabbit
Target Name: MARCH5
Sample Type: U937 Whole Cell lysates
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-SLC8A3 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: 721_B cell lysate -
Application
WB Suggested Anti-SULT6B1 Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate -
Application
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
Positive Control: Human heart -
Application
WB Suggested Anti-ZNF599 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 293T cell lysate -
Application
WB Suggested Anti-SSX4B Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: Human kidney -
Application
WB Suggested Anti-UBQLN1 Antibody Titration: 1 ug/ml
Positive Control: 293T cells lysate -
Application
Immunohistochemistry with Human Lung, respiratory epethelium tissue at an antibody concentration of 5.0ug/ml using anti-UBQLN1 antibody (ARP54958_P050).
-
Application
Anti-UBQLN1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. UBQLN1 Antibody ARP54958_P050 concentration 5 ug/ml.
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Application
WB Suggested Anti-Vwa5a Antibody
Titration: 1.0 ug/ml
Positive Control: Mouse Kidney -
Application
Host: Rabbit
Target Name: SPATA2
Sample Type: HCT15 Whole Cell lysates
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-ADAM8 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: PANC1 cell lysate -
Application
WB Suggested Anti-ORMDL3 Antibody
Titration: 1.0 ug/ml
Positive Control: Jurkat Whole Cell -
Application
Host: Rabbit
Target Name: NR4A1
Sample Tissue: Human Hela
Antibody Dilution: 1.0ug/ml -
Application
Rabbit Anti-NR4A1 Antibody
Catalog Number: ARP31941
Paraffin Embedded Tissue: Human Stomach
Cellular Data: Mucous Cells
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X -
Application
Rabbit Anti-NR4A1 Antibody
Catalog Number: ARP31941
Paraffin Embedded Tissue: Human Liver
Cellular Data: Hepatocytes
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X -
Application
Lanes:
Lane 1: 50ug mouse embryonic fibroblast lysate
Lane 2: 50ug mouse PTRF-KO embryonic fibroblast lysate
Lane 3: 50ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Donkey anti-Rabbit-HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
PTRF
Submitted by:
Libin Liu, Boston University. -
Application
Host: Rabbit
Target Name: PTRF
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml -
Application
IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.
-
Application
Lanes:
Lane 1: 5 ug 293T cell lysate
Lane 2: 10 ug 293T cell lysate
Lane 3: 20 ug 293T cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit HRP
Secondary Antibody Dilution:
1:10000
Gene Name:
HMGA2
Submitted by:
AnonymousHMGA2 is supported by BioGPS gene expression data to be expressed in HEK293T
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Application
WB Suggested Anti-TRIM32 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:62500
Positive Control: Jurkat cell lysate -
Application
PKLR antibody - N-terminal region (ARP41699_T100) validated by WB using HepG2 cell lysate at 1.0ug/ml.
There is BioGPS gene expression data showing that PKLR is expressed in HepG2
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Application
Rabbit Anti-PKLR Antibody
Catalog Number: ARP41699
Paraffin Embedded Tissue: Human Kidney
Cellular Data: Epithelial cells of renal tubule
Antibody Concentration: 4.0-8.0 ug/ml
Magnification: 400X -
Application
Sample Type: Equine Cartilage Explants
Primary Dilution: 1:800
Secondary Antibody: Bio-Rad 170-5046
Secondary Dilution::100,000
Image Submitted By: Adam Williams
University of Nottingham -
Application
WB Suggested Anti-MMP1 Antibody
Positive Control: Lane 1: 15ug HT29 cell lysate Lane 2: 15ug HT29 cell lysate
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:2000
Submitted by: Zhongsheng Peng, School of Medicine, University of Maryland Baltimore -
Application
Host: Rabbit
Target Name: MMP1
Sample Tissue: Human 293T
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: MARCH5
Sample Type: Human Fetal Kidney
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: G6pc
Sample Tissue: Mouse Lung
Antibody Dilution: 1.0ug/ml -
Application
Host: Rabbit
Target Name: G6PC
Sample Tissue: Mouse Testis
Antibody Dilution: 1ug/ml -
Application
Host: Rabbit
Target Name: SPPL2B
Sample Type: 293T Whole cell lysates
Antibody Dilution: 1.0ug/ml -
Application
WB Suggested Anti-TMTC1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:312500
Positive Control: Human Spleen -
Application
WB Suggested Anti-NUP35 Antibody Titration: 0.2-1 ug/ml
Positive Control: Jurkat cell lysate -
Application
WB Suggested Anti-Amigo2 Antibody
Titration: 1.0 ug/ml
Positive Control: Rat Heart -
Application
WB Suggested Anti-ACO2 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: 721_B cell lysateACO2 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells
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Application
ACO2 antibody - N-terminal region (ARP56301_P050) validated by WB using Proximal kidney tubules purfied from cortex at 5.0ug/ml.
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Application
WB Suggested Anti-FFAR1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Human Liver -
Application
WB Suggested Anti-GLIS2 Antibody Titration: 2.5ug/ml
ELISA Titer: 1:12500
Positive Control: HepG2 cell lysate -
Application
WB Suggested Anti-RBM14 Antibody Titration: 1.25ug/ml
ELISA Titer: 1:312500
Positive Control: SP2/0 cell lysate -
Application
Lanes:
Lane 1: 50ug Hela Lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-rabbit-HRP
Secondary Antibody Dilution:
1:10,000
Gene Name:
RBM14
Submitted by:
Archa Fox, University of Western Australia
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
