Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Instruction Manual!

Product name:	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)
Source:	Goat
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	ChIPFCIFIHCIPWB
Storage:	Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
UOM:	100ug

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Catalog Number：IC296276

Product Profile

Product Name	Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors. More

Product Name

Anti-mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								Anti-Mouse IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
							
More

Key Feature

Clonality	Monoclonal
Isotype	Cocktail
Host Species	Goat
TestedApplications	ChIPFCIFIHCIPWB
	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.: More
Species Reactivity	Mouse
Concentration	1mg/ml
Purification	Affinity purified

Target Information

TissueSpecificity	F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against human IgG and human serum.

Database Links

Application

Application

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (blue). Red = propidium iodide (fluorescent DNA dye).
Application

Flow cytometric analysis of untreated Jurkat cells using Akt Mouse mAb detected with Anti-Mouse IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).

ApplicationNotes	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.: More

Additional Information

StorageInstructions	Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.