Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

Instruction Manual!

Product name:	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)
Source:	Goat
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	ChIPFCIFIHCIPWB
Storage:	Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
UOM:	100ug

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

Catalog Number：IC296280

Product Profile

Product Name	Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)
AntibodyType	Secondary Antibodies
Productdescription	Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors. More

Product Name

Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate)

AntibodyType

Secondary Antibodies

Productdescription

							
								Anti-Rabbit IgG (H+L) F(ab')2 Fragment was conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
							
More

Key Feature

Clonality	Monoclonal
Isotype	Cocktail
Host Species	Goat
TestedApplications	ChIPFCIFIHCIPWB
	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.: More
Species Reactivity	Rabbit
Concentration	1mg/ml
Purification	Affinity purified

Target Information

TissueSpecificity	F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins and purified human paraproteins.

Database Links

Application

Application

Confocal immunofluorescent analysis of P19 cells using LIN28A Antibody detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5 ® (fluorescent DNA dye).
Application

Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002, Wortmannin and U0126 (blue), using Phospho-Akt (Ser473) (D9E) Rabbit mAb detected with Anti-Rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) and compared to a nonspecific negative control antibody (red).

ApplicationNotes	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays.: More

Additional Information

Form	Liquid
StorageInstructions	Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.