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NRF1 Antibody (monoclonal) (M01)
Instruction Manual!
| Product name: | NRF1 Antibody (monoclonal) (M01) |
| Source: | Mouse |
| Purity: | >95% |
| Buffer Formulation: | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
| Applications: | WB |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
NRF1 Antibody (monoclonal) (M01)
Catalog Number：IC215665
Product Profile
| Product Name | NRF1 Antibody (monoclonal) (M01) |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2F9 |
| Host Species | Mouse |
| Tested Applications | |
|
WB~~1:500~1000:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| Alternative Names |
ALPHAPALnuclear respiratory factor 1
|
|---|---|
| MolecularWeight(MW) | 53541 Da |
| Tissue Specificity | NRF1 (NP_005002, 201 a.a. ~ 286 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. |
Database Links
| Entrez Gene | 4899 |
|---|---|
| Nucleotide Accession | NP_005002 |
Application
-
Application
Western blot analysis of NRF1 over-expressed 293 cell line, cotransfected with NRF1 Validated Chimera RNAi ( Cat # H00004899-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with NRF1 monoclonal antibody (M01), clone 2F9 (Cat # AT3114a ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
-
Application
Western Blot analysis of NRF1 expression in transfected 293T cell line by NRF1 monoclonal antibody (M01), clone 2F9.Lane 1: NRF1 transfected lysate(55.7 KDa).Lane 2: Non-transfected lysate.
| Application Notes |
WB~~1:500~1000:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Clear, colorless solution in phosphate buffered saline, pH 7.2 . |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
