MET/HGFR Antibody

Instruction Manual!

Product name:	MET/HGFR Antibody
Source:	Mouse
Purity:	>95%
Buffer Formulation:	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Applications:	IFWB
Storage:	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:	100ug

MET/HGFR Antibody

Catalog Number：IC213617

Product Profile

Product Name	MET/HGFR Antibody
Antibody Type	Primary Antibodies

Key Feature

Clonality	Monoclonal
Isotype	IgG1
Clone Number	4AT247
Host Species	Mouse
Tested Applications	IFWB
	IF~~1:100 WB~~1:100~500:
Species Reactivity	Human
Concentration	1mg/ml
Purification

Target Information

Alternative Names	MET Hepatocyte growth factor receptor HGF/SF receptor Proto-oncogene c-Met Scatter factor receptor Tyrosine-protein kinase Met More
MolecularWeight(MW)	155541 Da
Function	Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1 Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of muscles and neuronal precursors, angiogenesis and kidney formation. In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells More
Tissue Specificity	Expressed in normal hepatocytes as well as in epithelial cells lining the stomach, the small and the large intestine. Found also in basal keratinocytes of esophagus and skin. High levels are found in liver, gastrointestinal tract, thyroid and kidney. Also present in the brain
Cellular Localization	Membrane; Single-pass type I membrane protein

Database Links

Entrez Gene	4233

Application

Application

Fluorescent confocal image of HepG2 cells stained with MET/HGFR antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AM1005a MET/HGFR primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the MET immunosignal to the cytoplasm, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000105976).
Application

Detection of endogenous Met in HepG2 cell line. 10 ug/lane of HepG2 cell lysate was used to examine the expression of human Met. Lanes 1-5 represent Abgent s different anti-Met monoclonal antibodies that are Cat# AM1001-1005. Lane 6 represents auto-phosohorylated-Met in HepG2 cell line detected by anti-phospho-Met Mab (Cat# AM1000).

Application Notes	IF~~1:100 WB~~1:100~500:

Additional Information

Form	Liquid
StorageInstructions	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.