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MAP2K2 Antibody
Instruction Manual!
| Product name: | MAP2K2 Antibody |
| Source: | Mouse |
| Purity: | >95% |
| Buffer Formulation: | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
| Applications: | WBIFFC |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
MAP2K2 Antibody
Catalog Number：IC213143
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Product Profile
| Product Name | MAP2K2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 7F5 |
| Host Species | Mouse |
| Tested Applications | |
|
WB~~1:500 - 2000. IF~~1:200 - 1000. FC~~1:200 - 400.:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| Gene Synonyms |
MEK2
More
MKK2 PRKMK2 |
|---|---|
| Alternative Names |
MEK2
More
MKK2 MAPKK2 |
| MolecularWeight(MW) | 44424 Da |
| Function |
Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity)
More
|
| Tissue Specificity | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
Database Links
| Entrez Gene | 5605 |
|---|---|
| Nucleotide Accession | NP_109587.1 |
Application
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Application
Figure 1: Western blot analysis using MAP2K2 mouse mAb against PC-12 (1), Jurkat (2), Hela (3) and NIH/3T3 (4) cell lysate.
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Application
Figure 2:Immunofluorescence analysis of Hela cells using anti-MAP2K2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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Application
Figure 3: Flow cytometric analysis of Hela cells using anti-MAP2K2 mAb (green) and negative control (purple).
| Application Notes |
WB~~1:500 - 2000. IF~~1:200 - 1000. FC~~1:200 - 400.:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified recombinant fragment of human MAP2K2 expressed in E. Coli. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
