MDM4 Antibody

Instruction Manual!

Product name:	MDM4 Antibody
Source:	Mouse
Purity:	>95%
Buffer Formulation:	Purified recombinant fragment of human MDM4 expressed in E. Coli.
Applications:	WBIHCIF
Storage:	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
UOM:	100ug

Catalog Number：ic213472

Product Name	MDM4 Antibody
Antibody Type	Primary Antibodies

Gene Synonyms	MDMX
Alternative Names	HDMX MDMX MRP1 MDM4 More
MolecularWeight(MW)	54864 Da
Function	Inhibits p53/TP53- and TP73/p73-mediated cell cycle arrest and apoptosis by binding its transcriptional activation domain. Inhibits degradation of MDM2. Can reverse MDM2-targeted degradation of TP53 while maintaining suppression of TP53 transactivation and apoptotic functions More
Tissue Specificity	Expressed in all tissues tested with high levels in thymus
Cellular Localization	Nucleus.

Entrez Gene	4194
Nucleotide Accession	NP_002384.2

Application

Figure 1: Western blot analysis using anti-MDM4 mAb against Hela (1), A549 (2) and A431 (3) cell lysate.
Application

Figure 2: Immunohistochemical analysis of paraffin-embedded human cerebra (left) and lung carcinoma (right) tissues, showing nuclear localization with DAB staining using anti-MDM4 mAb.
Application

Figure 3: Confocal immunofluorescence analysis of Hela (left) and L-02 (right) cells using anti-MDM4 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin.

Application Notes	WB~~1:500 - 2000. IHC~~1:200 - 1000. IF~~1:200 - 1000.:

Form	Liquid
StorageInstructions	For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer	Purified recombinant fragment of human MDM4 expressed in E. Coli.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.