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Ezrin (phospho Tyr353) Antibody

Ezrin (phospho Tyr353) Antibody Ezrin (phospho Tyr353) Antibody

Instruction Manual!

Product name: Ezrin (phospho Tyr353) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Ezrin (phospho Tyr353) Antibody

Catalog Number:IC278349

Product Profile

Product Name Ezrin (phospho Tyr353) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.1) Zhao H, et al. (2004) Proc Natl Acad Sci U S A101 (25): 9485-9490. Wang Q, et al. (2003) J Biol Chem 278 (48): 47731-47743. Gautreau A, et al. (1999) Proc Natl Acad Sci U S A 96 (13): 7300-7305. Crepaldi T, et al. (1997) J Cell Biol 138 (2): 423-34.
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Immunogen
Ezrin (Phospho-Tyr353) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 353 (Q-D-Y (p) -E-E) derived from Human EZRIN.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

HumanMouse

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol EZR
Alternative Names
CVL
CVIL
VIL2
HEL-S-105
ESR
NR3A1
Cytovillin
EZRI
Villin 2
p81
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MolecularWeight(MW)  81 kDa
Tissue Specificity This antibody detects endogenous level of Ezrin only when phosphorylated at tyrosine 353.

Database Links

Entrez Gene 7430
Protein Accession NP_001104547.1

Application

  • Application

    Western blot analysis of lysed extracts from 293 cells untreated or treated with EGF using Ezrin (Phospho-Tyr353).

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Ezrin (Phospho-Tyr353).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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