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Pyk2 (phospho Tyr402) Antibody

Pyk2 (phospho Tyr402) Antibody Pyk2 (phospho Tyr402) Antibody

Instruction Manual!

Product name: Pyk2 (phospho Tyr402) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

Pyk2 (phospho Tyr402) Antibody

Catalog Number:IC289329

Product Profile

Product Name Pyk2 (phospho Tyr402) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
							
Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. May represent an important signaling intermediate between neuropeptide activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. Involved in osmotic stress-dependent SNCA 'Tyr-125' phosphorylation.1) Gluck SL, et al. (2004) J Clin Invest; 114 (12): 1696-1699Benzing T, et al. (2001) Proc Natl Acad Sci U S A; 98 (17): 9784-9789Tian D, et al. (2002) Mol Cell Biol; 22 (8): 2650-2662Lu Z, et al. (2001) Mol Cell Biol; 21 (12): 4016-4031Krishnan HH, et al. (2006) J Virol; 80 (3): 1167-1180
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Immunogen
Pyk2 (Phospho-Tyr402) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 402 (D-I-Y (p) -A-E) derived from Human Pyk2.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol PTK2B
Alternative Names
PKB
PTK
CAKB
FAK2
PYK2
CADTK
FADK2
RAFTK
MAPTL
MTBT1
TAU
FADK 2
Focal adhesion kinase 2
PTK2B
Proline-rich tyrosine kinase 2 RAFTK
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MolecularWeight(MW)  116 kDa
Tissue Specificity This antibody detects endogenous level of Pyk2 only when phosphorylated at tyrosine 402.

Database Links

Entrez Gene 2185
Protein Accession NP_004094.3

Application

  • Application

    Western blot analysis of lysed extracts from 293 cells untreated or treated with Serum using Pyk2 (Phospho-Tyr402).

  • Application

    Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Pyk2 (Phospho-Tyr402). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using Pyk2 (Phospho-Tyr402).

ApplicationNotes
 Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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