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IkB a (phospho Ser32 / Ser36) Antibody
Instruction Manual!
| Product name: | IkB a (phospho Ser32 / Ser36) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
IkB a (phospho Ser32 / Ser36) Antibody
Catalog Number：IC281776
Product Profile
| Product Name | IkB a (phospho Ser32 / Ser36) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
IkB-a (Phospho-Ser32/Ser36) antibody was raised against a peptide sequence around phosphorylation site of serine 32/36 (H-D-S (p) -G-L-D-S (p) -M-K) derived from Human IkappaB-α. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | NFKBIA |
|---|---|
| Alternative Names |
IKBA
More
MAD-3 NFKBI ADDA I-kappa-B-alpha IKBA NF-kappaB inhibitor alpha NFKBIA |
| MolecularWeight(MW) | 39 kDa |
| Tissue Specificity | This antibody detects endogenous level of IkappaB-α only when phosphorylated at serine 32/36. |
Database Links
| Entrez Gene | 4792 |
|---|---|
| Protein Accession | NP_065390.1 |
Application
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkappaB-α (Phospho-Ser32/Ser36). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using IkappaB-α (Phospho-Ser32/Ser36).
-
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with TNF using IκB-α (Phospho-Ser32/Ser36).
| ApplicationNotes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
