Amyloid beta A4 (phospho Thr743/668) Antibody

Instruction Manual!

Product name:	Amyloid beta A4 (phospho Thr743/668) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	FIHCWB
Storage:	Store antibody at -20˚C for up to one year.
UOM:	100ug

Catalog Number：IC271044

Product Name	Amyloid beta A4 (phospho Thr743/668) Antibody
Antibody Type	Primary Antibodies
Productdescription	Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibits Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(O) and JIP. Inhibits G(o) alpha ATPase activity By similarity. Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1.1) Ming-Sum Lee J. Cell Biol., Oct 2003; 163: 83. More
Immunogen	Amyloid beta A4 (phospho Thr743/668) antibody was raised against a peptide sequence around phosphorylation site of threonine 743 /668 (A-V-T (p)-P-E) derived from Human Amyloid beta A4.

Clonality	Polyclonal
Isotype	IgG
Host Species	Rabbit
TestedApplications	IFIHCWB
	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
Species Reactivity	HumanMouseRat
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	APP
Alternative Names	A4 AD1 APP APPI Beta-amyloid protein 42 More
MolecularWeight(MW)	140 kDa
Tissue Specificity	The antibody detects endogenous levels of Amyloid beta A4 only when phosphorylated at threonine 743/668.

Entrez Gene	351
Protein Accession	NP_000475.1

Application

Immunofluorescence staining of methanol-fixed HeLa cells using Amyloid beta A4 (phospho-Thr743/668) Antibody.
Application

Western blot analysis of extracts from cos-7 cells (Lane 2), using Amyloid beta A4 (Phospho-Thr743/668) Antibody. The lane on the left is treated with antigen-specific peptide.
Application

Immunohistochemical analysis of paraffin-embedded human brain tissue using Amyloid beta A4 (phospho-Thr743/668) antibody (left) or the same antibody preincubated with blocking peptide (right).

ApplicationNotes	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:

Form	Liquid
StorageInstructions	Store antibody at -20˚C for up to one year.
Storage Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.