FGFR1 Antibody

Instruction Manual!

Product name:	FGFR1 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIHC-PWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC278816

Product Name	FGFR1 Antibody
Antibody Type	Primary Antibodies
Productdescription	FGFR1 is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction.1) Jiao, J., et al., Arch. Biochem. Biophys. 410(2):187-200 (2003). Fu, L., et al., J. Comp. Neurol. 462(2):265-273 (2003). Lundin, L., et al., Exp. Cell Res. 287(1):190-198 (2003). Kiselyov, V.V., et al., Structure (Camb.) 11(6):691-701 (2003). Baumann, H., et al., J. Biol. Chem. 278(18):16198-16208 (2003). More
Immunogen	This FGFR1 antibody is generated from rabbits immunized with a His fusion protein from human FGFR1.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIHC-PWB
	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Unpurified

Gene Symbol	FGFR1
Alternative Names	Fibroblast growth factor receptor 1 FGFR-1 Basic fibroblast growth factor receptor 1 BFGFR bFGF-R-1 Fms-like tyrosine kinase 2 FLT-2 N-sam Proto-oncogene c-Fgr CD331 FGFR1 BFGFR CEK FGFBR FLG FLT2 HBGFR More
Molecular Weight(MW)	92 kDa

Entrez Gene	2260
Protein Accession	P11362

Application

Flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

FGFR1 Antibody IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application

Western blot analysis of FGFR1 Antibody in mouse liver tissue lysates (35ug/lane)

ApplicationNotes	For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.