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p44 / 42 MAP Kinase (phospho Tyr204) Antibody
Instruction Manual!
| Product name: | p44 / 42 MAP Kinase (phospho Tyr204) Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | IFIHCWB |
| Storage: | Store antibody at -20˚C for up to one year. |
| UOM: | 100ug |
p44 / 42 MAP Kinase (phospho Tyr204) Antibody
Catalog Number：IC287172
Product Profile
| Product Name | p44 / 42 MAP Kinase (phospho Tyr204) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Productdescription |
|
| Immunogen |
p44/42 MAP Kinase (Phospho-Tyr204) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 204 (T-E-Y (p) -V-A) derived from Human p44/42 MAP Kinase. |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| TestedApplications | |
|
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | MAPK3 |
|---|---|
| Alternative Names |
ERK1
More
ERT2 ERK-1 PRKM3 P44ERK1 P44MAPK HS44KDAP HUMKER1A p44-ERK1 p44-MAPK PKC2 ERK Extracellular signal- regulated kinase 1 Insulin-stimulated MAP2 kinase MAP kinase 1 |
| MolecularWeight(MW) | 42, 44 kDa |
| Tissue Specificity | This antibody detects endogenous level of p44/42 MAP Kinase only when phosphorylated at tyrosine 204. |
Database Links
| Entrez Gene | 5595 |
|---|---|
| Protein Accession | NP_001035145.1 |
Application
-
Application
Western blot analysis of lysed extracts from 293 cells untreated or treated with UV using p44/42 MAP Kinase (Phospho-Tyr204).
-
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p44/42 MAP Kinase (Phospho-Tyr204). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells showing centrosome and nuclear staining using p44/42 MAP Kinase (Phospho-Tyr204).
| Application Notes |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
