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CDK1/CDC2 (phospho Thr14) Antibody

CDK1/CDC2 (phospho Thr14) Antibody CDK1/CDC2 (phospho Thr14) Antibody

Instruction Manual!

Product name: CDK1/CDC2 (phospho Thr14) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:WB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

CDK1/CDC2 (phospho Thr14) Antibody

Catalog Number:IC275131

Product Profile

Product Name CDK1/CDC2 (phospho Thr14) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus.1) Sarkar S, Dey BK, Dutta A (2010) Mol Biol Cell 21, 2138-49
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Immunogen
CDK1/CDC2 (phospho Thr14) antibody was raised against a peptide sequence around phospho rylation site of threonine14 (E-G-T (p)-Y-G) derived from Human CDK1/CDC2 .

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
Tested Applications

WB

Western Blot: 1:500~1:1000:
Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CDK2
Alternative Names
CDC28
CDC2A
CDK1
MPF
kinase Cdc2
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MolecularWeight(MW)  34 kDa
Tissue Specificity The antibody detects endogenous level of CDK1/CDC2 only when phosphorylated at threonine 14.

Database Links

Entrez Gene 1017
Protein Accession NP_001277159.1

Application

  • Application

    Western blot analysis of extracts from HepG2 cells treated with Forskolin using Phospho-CDK1/CDC2 (Thr14) Antibody. The lane on the right is treated with the antigen-specific peptide.

Application Notes
Western Blot: 1:500~1:1000:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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