MAPK14 Antibody

Instruction Manual!

Product name:	MAPK14 Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	Supplied in PBS with 0.09% (W/V) sodium azide.
Applications:	FACSIFWB
Storage:	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:	100ug

Catalog Number：IC284159

Product Name	MAPK14 Antibody
Antibody Type	Primary Antibodies
Productdescription	P38 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.1) Cheung, P.C., et al., EMBO J. 22(21):5793-5805 (2003). More
Immunogen	This MAPK14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 295-324 amino acids from the C-terminal region of human MAPK14.

Clonality	Polyclonal
Isotype	Ig
Host Species	Rabbit
TestedApplications	FACSIFWB
	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:
Species Reactivity	Human
Concentration	1mg/ml
Purification	Affinity purified

Gene Symbol	MAPK14
Alternative Names	Mitogen-activated protein kinase 14 MAP kinase 14 MAPK 14 Cytokine suppressive anti-inflammatory drug-binding protein CSAID-binding protein CSBP MAP kinase MXI2 MAX-interacting protein 2 Mitogen-activated protein kinase p38 alpha MAP kinase p38 al More
Molecular Weight(MW)	41 kDa
Sequence Similarities	Predicted species reactivity based on immunogen sequence: Mouse

Entrez Gene	1432
Protein Accession	Q16539

Application

Confocal immunofluorescent analysis of MAPK14 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application

Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application

Antibody is used in Western blot to detect P38 in Jurkat cell lysate.
Application

Western blot analysis of P38 using P38 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK14 gene (Lane 2).

ApplicationNotes	For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50:

Form	Liquid
StorageInstructions	Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer	Supplied in PBS with 0.09% (W/V) sodium azide.