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STAT1 alpha Antibody

STAT1 alpha Antibody STAT1 alpha Antibody

Instruction Manual!

Product name: STAT1 alpha Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Applications:ELISAICCIHC-PIPWB
Storage:STAT1 alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
UOM:100ug

STAT1 alpha Antibody

Catalog Number:IC292322

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Product Profile

Product Name STAT1 alpha Antibody
Antibody Type Primary Antibodies
Productdescription 
							
STAT1 alpha Antibody: STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (Stat1, Stat2, Stat3, Stat4, Stat5a, Stat5b, and Stat6), Stat1, Stat3, Stat5a, and Stat5b have a wide activation profile. STAT1 is activated by many different ligands including IFN family (IFN-α, IFN-β, IFN-γ and IL-10), gp130 family (IL-6, IL-11, LIF, CNTF, and G-CSF), and receptor tyrosine kinases (EGF, PDGF, and CSF-1).1) Leonard WJ and O'Shea JJ. Jaks and STATs: biological implications. Annu. Rev. Immunol. 1998; 16:293-322.
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Immunogen
STAT1 alpha antibody was raised against a peptide corresponding to 38 amino acids near the carboxy terminus of human STAT1 alpha. The sequences differ from the murine corresponding sequences by four amino acids. The immunogen is located within the last 50 

Key Feature

Clonality Polyclonal
Isotype IgG
Host Species Rabbit
TestedApplications

 ELISAICCIHC-PIPWB

STAT1 alpha antibody can be used for Western blot at 0.5 - 1 μg and for immunoprecipitation at 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL.:
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Species Reactivity

HumanMouseRat

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol STAT1
Alternative Names
STAT1 alpha Antibody: CANDF7
ISGF-3
STAT91
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MolecularWeight(MW)  Predicted: 91 kDa Observed: 90 kDa
Sequence Similarities 4) Cat. No. 1204 - K562 Cell Lysate
Tissue Specificity At least two isoforms of STAT1 are known to exist; this antibody will only recognize the larger isoform.

Database Links

Entrez Gene 6772
Protein Accession NP_009330

Application

  • Application

    Immunocytochemistry of STAT1 alpha in HeLa cells with STAT1 alpha antibody at 10 μg/mL.

  • Application

    Immunohistochemistry of STAT1alpha in human colon tissue with STAT1alpha antibody at 2.5 μg/ml.

  • Application

    Western blot analysis of STAT1 alpha in whole cell lysates from HeLa (H), Jurkat (J), A431 (A), K562 (K), and NIH3T3 (N) cells, with STAT1a antibody at 1 μg/mL.

PositiveControl 1) Cat. No. 1201 - HeLa Cell Lysate
ApplicationNotes 
STAT1 alpha antibody can be used for Western blot at 0.5 - 1 μg and for immunoprecipitation at 2 to 4 μg per sample. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL.:
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Additional Information

Form Liquid
StorageInstructions  STAT1 alpha antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Storage Buffer STAT1 alpha Antibody is supplied in PBS containing 0.02% sodium azide.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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