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CREB (phospho Ser133) Antibody

CREB (phospho Ser133) Antibody CREB (phospho Ser133) Antibody

Instruction Manual!

Product name: CREB (phospho Ser133) Antibody
Source:Rabbit
Purity:>95%
Buffer Formulation:Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:IHCWB
Storage:Store antibody at -20˚C for up to one year.
UOM:100ug

CREB (phospho Ser133) Antibody

Catalog Number:IC276095

Product Profile

Product Name CREB (phospho Ser133) Antibody
Antibody Type Primary Antibodies
Productdescription 
							
CREB1 is a transcription factor that is a member of the bZIP family of DNA binding proteins. It binds as a homodimer to the CRE (cAMP-Responsive Element), an octameric palindrome containing a conserved core sequence, 5-prime-TGACG-3-prime. It consists of two transcript variants encoding respective isoforms produced by alternate splicing. It is mapped to 2q32.3-q34 . It is phosphorylated by several protein kinases and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. CREB1 is crucial for the consolidation of long-term conditioned fear memories, but not for encoding, storage, or retrieval of these memories. It is required for the stability of reactivated or retrieved conditioned fear memories .1) Xing J, et al. (1998) Mol Cell Biol 18 (4): 1946-55. Tan Y, et al. (1996) EMBO J; 15 (17): 4629-42. Hao, M. et al. (1996) J. Biol. Chem. 271, 29380-29385. Mayo LD, et al. (2001) Biol Chem; 276 (27): 25184-9.
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Immunogen
CREB (Phospho-Ser133) antibody was raised against a peptide sequence around phosphorylation site of serine 133 (R-P-S (p) -Y-R) derived from Human CREB.

Key Feature

Clonality Polyclonal
Host Species Rabbit
Tested Applications

IHCWB

Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:
Species Reactivity

Human

Concentration 1mg/ml
Purification Affinity purified

Target Information

Gene Symbol CREB1
Alternative Names
CREB
CCNB
CREB-1
CREB1
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Molecular Weight(MW) 43 kDa
Tissue Specificity This antibody detects endogenous level of CREB only when phosphorylated at serine 133.

Database Links

Entrez Gene 1385
Protein Accession NP_004370.1

Application

  • Application

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CREB (Phospho-Ser133).

  • Application

    Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using CREB (Phospho-Ser133).

Application Notes
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100:

Additional Information

Form Liquid
StorageInstructions  Store antibody at -20˚C for up to one year.
Storage Buffer Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

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