p53 (phospho Ser392) Antibody

Instruction Manual!

Product name:	p53 (phospho Ser392) Antibody
Source:	Rabbit
Purity:	>95%
Buffer Formulation:	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.
Applications:	WB
Storage:	For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
UOM:	100ug

p53 (phospho Ser392) Antibody

Catalog Number：IC287192

Product Profile

Product Name	p53 (phospho Ser392) Antibody
Antibody Type	Primary Antibodies
Productdescription	p53 has a well established role in the blocking the proliferative action of damaged cells and acting in essence as an anticancer agent. It has been called the guardian of the genome. Phosphorylation of Ser392 in p53 is associated with formation of human tumors. In addition p53 has also been linked to affects of aging and oxidative stress. An increase in p53 has also been linked to deficits in LTP and learning and memory. We raised this polyclonal antibody against a peptide representing the sequence around Ser392 of p53.1) Saito, S., Yamaguchi, H., Higashimoto, Y., Chao, C., Xu, Y., Fornace, A.J., Jr., Appella, E., and Anderson, C.W., "Phosphorylation site interdependence of human p53 post-translational modifications in response to stress," J. Biol. Chem., 278 (2003) 37,536-37,544. More
Immunogen	p53 (Ser392) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phospho-Ser392 of p53.

Key Feature

Clonality	Polyclonal
Host Species	Rabbit
TestedApplications	WB
	Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: More
Species Reactivity	HumanMonkey
Concentration	1mg/ml
Purification	Affinity purified

Target Information

Gene Symbol	TP53
Alternative Names	P53 BCC7 LFS1 TRP53 P53 Antigen NY-CO-13 More
Molecular Weight(MW)	53
Tissue Specificity	p53 antibody is specific for the 53 kDa p53 protein phosphorylated at Ser392.

Database Links

Entrez Gene	7157
Protein Accession	P04637

Application

Application

Western blot of rat brain fraction lysate showing phosphospecific immunolabeling of the ~53k p53 protein phosphorylated at Ser392.

ApplicationNotes	Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.: More

ApplicationNotes

Immunolabeling of the 53 kDa p53 protein in Western blots of PMA treated NIH3T3 cells is blocked by the Ser392 phosphopeptide used as antigen but by the corresponding non-phosphopeptide. Applications include Dot Blots (DB) and Western Blots (WB). Immunohistochemistry: not determined. Human and some non-human primates have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.:

Form	Liquid
StorageInstructions	For long term storage –80˚C is recommended, but shorter term storage at –20˚C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stable for one year.
Storage Buffer	100 uL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per mL BSA and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.

p53 (phospho Ser392) Antibody

p53 (phospho Ser392) Antibody

Product Profile

Key Feature

Target Information

Database Links

Application

Application

Additional Information