Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate)

Instruction Manual!

Product name:	Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate)
Source:	Rat
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WBIPIHCIFFCChIP
Storage:	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied i
UOM:	100ug

Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate)

Catalog Number：IC296303

Product Profile

Product Name	Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-rat IgG (H+L) was conjugated to Alexa Fluor^® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml.

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Rat
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.

Database Links

Application

Application

Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate) (blue) and COX IV (3E11) Rabbit mAb detected with Anti-rabbit IgG (H+L), F(ab') 2 Fragment (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with DY-554 phalloidin (red).
Application

Flow cytometric analysis of untreated HeLa cells using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 647 Conjugate) (green) and compared to a nonspecific negative control antibody (red).

Additional Information

StorageInstructions	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageInstructions

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution of 1:250 - 1:1000 should yield acceptable results for immunofluorescent and flow cytometry assays. Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.