Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)

Instruction Manual!

Product name:	Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)
Source:	Rat
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WBIPIHCIFFCChIP
Storage:	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays: Immunofluorescent: 1:500
UOM:	100ug

Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)

Catalog Number：IC296305

Product Profile

Product Name	Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-rat IgG (H+L) antibody was conjugated to Alexa Fluor^® 488 fluorescent dye under optimal conditions and formulated at 2 mg/ml.

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Rat
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.

Database Links

Application

Application

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or heat shocked (right), using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green). Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red).
Application

Flow cytometric analysis of HeLa cells using HSP70 (6B3) Rat mAb detected with Anti-rat IgG (H+L), (Alexa Fluor ® 488 Conjugate) (green) and compared to a nonspecific negative control antibody (red).

Additional Information

StorageInstructions	The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays: Immunofluorescent: 1:500 - 1:2000 Flow Cytometry: 1:500 - 1:1000 Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Storage Buffer	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

StorageInstructions

The optimal dilution of the anti-species antibody should be determined for each primary antibody by titration. However, a final dilution shown below should yield acceptable results for immunofluorescent and flow cytometry assays:

Immunofluorescent: 1:500 - 1:2000

Flow Cytometry: 1:500 - 1:1000

Storage: Supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, 5 mM sodium azide. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.