Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

Instruction Manual!

Product name:	Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)
Source:	Biotin
Purity:	>95%
Buffer Formulation:	phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applications:	WBIPIHCIFFCChIP
Storage:	Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
UOM:	100ug

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

Catalog Number：IC296294

Product Profile

Product Name	Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)
Antibody Type	Secondary Antibodies
Productdescription	Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. This product has been optimized to detect biotinylated primary antibodies. HRP conjugated antibodies do not require incubation with a secondary antibody. *Do not mix this antibody in solution with any Anti-rabbit antibody. Anti-rabbit antibodies will cross react with this antibody and could result in decreased activity of both Anti-rabbit and Anti-biotin (D5A7) Rabbit mAb. More

Product Name

Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate)

Antibody Type

Secondary Antibodies

Productdescription

							
								
								
									Anti-biotin (D5A7) Rabbit mAb is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. This product has been optimized to detect biotinylated primary antibodies. HRP conjugated antibodies do not require incubation with a secondary antibody. *Do not mix this antibody in solution with any Anti-rabbit antibody. Anti-rabbit antibodies will cross react with this antibody and could result in decreased activity of both Anti-rabbit and Anti-biotin (D5A7) Rabbit mAb.
								

							
More

Key Feature

Clonality	Monoclonal
Tested Applications	WBIPIHCIFFCChIP
Species Reactivity	Biotin
Concentration	1mg/ml
Purification

Target Information

TissueSpecificity	Anti-biotin (D5A7) Rabbit mAb (HRP Conjugate) recognizes biotin attached to proteins, peptides, oligonucleotides and solid matrices. Species Reactivity: All Species Expected

Database Links

Application

Application

Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine (1 μM, 3 hr) using Cleaved PARP (Asp214) (D64E10) XP ® Rabbit mAb (Biotinylated) primary antibody and Anti-Biotin (D5A7) Rabbit mAb (HRP Conjugate) as secondary detection antibody.

Additional Information

StorageInstructions

Application	Dilutions
Western Blotting	1:1000

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA), and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Storage Buffer

phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.