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HA Tag Antibody
Instruction Manual!
| Product name: | HA Tag Antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Applications: | WB IF |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
HA Tag Antibody
Catalog Number：IC234703
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Product Profile
| Product Name | HA Tag Antibody |
|---|---|
| Antibody Type | Tags Antibodies |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | |
|
WB~~1:50~100 IF~~1:10~50 CHIP~~1:100:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| MolecularWeight(MW) | 25712 Da |
|---|---|
| Tissue Specificity | KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen. |
Database Links
Application
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Application
Western blot analysis of anti-HA tag pabin HA-tagged recombinant protein bacterial lysate.
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Application
GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))
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Application
ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))
| Application Notes |
WB~~1:50~100 IF~~1:10~50 CHIP~~1:100:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
