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c-Myc Antibody Phospho (pT58/pS62)
Instruction Manual!
| Product name: | c-Myc Antibody Phospho (pT58/pS62) |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
| Applications: | WB |
| Storage: | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| UOM: | 100ug |
c-Myc Antibody Phospho (pT58/pS62)
Catalog Number：IC227902
Product Profile
| Product Name | c-Myc Antibody Phospho (pT58/pS62) |
|---|---|
| Antibody Type | Tags Antibodies |
Key Feature
| Clonality | Monoclonal |
|---|---|
| Clone Number | E203 |
| Host Species | Rabbit |
| Tested Applications | |
|
WB~~1:1000:
|
|
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification |
Target Information
| GeneSynonyms |
BHLHE39
|
|---|---|
| Alternative Names |
MYC
More
Myc proto-oncogene protein c-Myc Transcription factor p64 |
| MolecularWeight(MW) | 48804 Da |
| Function |
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes
|
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Thr58 and Ser62 of human C-Myc was used as immunogen. The antibody only detects c-Myc phosphorylated on Threonine 58 and Serine 62. Predicted to cross-react with most species, based on sequence homology. |
| Cellular Localization | Nucleus, nucleoplasm. Nucleus, nucleolus. |
Database Links
| Entrez Gene | 4609 |
|---|
Application
-
Application
A. Western blot analysis on A431 cell lysate using anti-Phospho-c-Myc (pT58/pS62) RabMAb (Cat. #AJ1192c), dilution 1:1,000. Cell lysates were either (1) untreated or (2) treated with TPA.
-
Application
B. Immunofluorescent staining of A4321 cells using anti-phospho-c-Myc (T58/S62) RabMAb (Cat. #AJ1192c).
| Application Notes |
WB~~1:1000:
|
|---|
Additional Information
| Form | Liquid |
|---|---|
| StorageInstruction s | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
|
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