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PDK1 antibody
Instruction Manual!
| Product name: | PDK1 antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-PDK1 polyclonal antibody - middle region
Catalog Number：IC105471
Product Profile
| ProductName | Rabbit anti-PDK1 polyclonal antibody - middle region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-PDK1 antibody: synthetic peptide directed towards the middle region of human PDK1 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | PDK1 |
|---|---|
| Gene Synonyms |
-
|
| Gene Full Name | Pyruvate dehydrogenase kinase, isozyme 1 |
| Gene Summary |
Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation.Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
More
|
| MolecularWeight(MW) | 47kDa |
| Sequence | 436 amino acids |
Database Links
| Entrez Gene | 5163 |
|---|---|
| SwissProt ID | Q15118 |
| Protein Accession | NP_002601 |
Application
-
Western blot
Host: Rabbit
Target Name: CHAD
Sample Tissue: Human Adult Placenta
Antibody Dilution: 1.0ug/ml -
Western blot
0.2-1 ug/ml
ELISA Titer: 1:1562500
Positive Control: MCF7 cell lysate
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
