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ZEB1 antibody
Instruction Manual!
| Product name: | ZEB1 antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-ZEB1 polyclonal antibody - N-terminal region
Catalog Number：IC105248
Product Profile
| ProductName | Rabbit anti-ZEB1 polyclonal antibody - N-terminal region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-ZEB1 antibody: synthetic peptide directed towards the N terminal of human ZEB1 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | ZEB1 |
|---|---|
| Gene Synonyms |
AREB6
More
BZP DELTA-EF1 FECD6 MGC133261 NIL-2-A NIL-2A NIL2A TCF8 ZEB ZFHEP ZFHX1A PPCD3 DELTAEF1 |
| Gene Full Name | Zinc finger E-box binding homeobox 1 |
| Gene Summary |
ZEB1 is a zinc finger transcription factor that represses T-lymphocyte-specific IL2 gene expression by binding to a negative regulatory domain 100 nucleotides 5-prime of the IL2 transcription start site.
|
| Alternative Names |
AREB6
More
BZP DELTA-EF1 FECD6 MGC133261 NIL-2-A NIL-2A NIL2A TCF8 ZEB ZFHEP ZFHX1A PPCD3 DELTAEF1 |
| MolecularWeight(MW) | 124kDa |
| Sequence | 1124 amino acids |
Database Links
| Entrez Gene | 6935 |
|---|---|
| SwissProt ID | P37275 |
| Protein Accession | NP_110378 |
Application
-
Immunohistochemical
Human Capan1 cells (Pancreatic cancer cell line)
Primary Antibody Dilution :
1:300
Secondary Antibody :
Anti-rabbit-AlexaFluor-488
Secondary Antibody Dilution :
1:200
Color/Signal Descriptions :
ZEB: Green DAPI: Blue
Gene Name :
ZEB1
Submitted by :
Dr. Pankaj Singh, UNMC, Omaha, NE -
Immunoblot
1. 45ug capan1 cell lysate
2. 45 ug HPAF cell lysate
Primary Antibody Dilution:
1:1000
Secondary Antibody:
Anti-Rabbit HRP
Secondary Antibody Dilution:
1:5000
Gene Name:
ZEB1
Submitted by:
Dr. Pankaj Singh, UNMC, Omaha, NE
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
