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RAD17 antibody
Instruction Manual!
| Product name: | RAD17 antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-RAD17 polyclonal antibody - N-terminal region
Catalog Number：IC105192
Product Profile
| ProductName | Rabbit anti-RAD17 polyclonal antibody - N-terminal region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-RAD17 antibody: synthetic peptide directed towards the N terminal of human RAD17 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | RAD17 |
|---|---|
| Gene Synonyms |
CCYC
More
FLJ41520 HRAD17 R24L RAD17SP RAD24 |
| Gene Full Name | RAD17 homolog (S. pombe) |
| Gene Summary |
RAD17 is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by ATR after the damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation.
More
|
| Alternative Names |
CCYC
More
FLJ41520 HRAD17 R24L RAD17SP RAD24 |
| MolecularWeight(MW) | 76kDa |
| Sequence | 670 amino acids |
Database Links
| Entrez Gene | 5884 |
|---|---|
| SwissProt ID | O75943-2 |
| Protein Accession | NP_002864 |
Application
-
Western blot
Positive Control: Lane1: 25ug HeLa lysate, Lane2: 25ug Xenopus laevis egg extract, Lane3: 25ug mouse embryonic stem cell lysate, Lane4: 25ug HEK293T lysate
Primary Antibody Dilution : 1:500
Secondary Antibody : Anti-rabbit-HRP
Secondry Antibody Dilution : 1:3000
Submitted by: Domenico Maiorano, Institute of Human Genetics, CNRS -
Western blot
1 ug/ml
Positive Control: Fetal liver cell lysate
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Note: The product is for research use only,not for use in diagnostic or therapeutic procedures.
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