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EEA1 antibody
Instruction Manual!
| Product name: | EEA1 antibody |
| Source: | Rabbit |
| Purity: | >95% |
| Buffer Formulation: | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Applications: | WB, IP |
| Storage: | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| UOM: | 100ug |
Rabbit anti-EEA1 polyclonal antibody - N-terminal region
Catalog Number：IC105120
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Product Profile
| ProductName | Rabbit anti-EEA1 polyclonal antibody - N-terminal region |
|---|---|
| AntibodyType | Primary Antibodies |
| Immunogen |
The immunogen for anti-EEA1 antibody: synthetic peptide directed towards the N terminal of human EEA1 |
Key Feature
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | |
| Species Reactivity | |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
Target Information
| Gene Symbol | EEA1 |
|---|---|
| Gene Synonyms |
EEA1
|
| Gene Full Name | Early endosome antigen 1 |
| Gene Summary |
EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25.
|
| Alternative Names |
MST105
ZFYVE2 MSTP105 |
| MolecularWeight(MW) | 162kDa |
| Sequence | 1411 amino acids |
Database Links
| Entrez Gene | 8411 |
|---|---|
| SwissProt ID | Q15075 |
| Protein Accession | NP_003557 |
Application
-
Application
Rabbit Anti-EEA1 Antibody
Catalog Number:
Formalin Fixed Paraffin Embedded Tissue: Human Adult Liver
Observed Staining: Cytoplasm in hepatocytes, moderate signal, moderate tissue distribution
Primary Antibody Concentration: 1:100
Secondary Antibody: Donkey anti-Rabbit-Cy3
Secondary Antibody Concentration: 1:200
Magnification: 20X
Exposure Time: 0.5 – 2.0 sec
Protocol located in Reviews and Data. -
Application
WB Suggested Anti-EEA1 Antibody Titration: 0.06ug/ml
ELISA Titer: 1:312500
Positive Control: Human Placenta -
Application
Amount and Sample Type :
500 ug rat brain homogenate
Amount of IP Antibody :
6 ug
Primary Antibody :
EEA1
Primary Antibody Dilution :
1:500
Secondary Antibody :
Goat anti-rabbit Alexa-Fluor 594
Secondary Antibody Dilution :
1:5000
Gene Name :
EEA1
Submitted by :
Dr. Yuzhi Chen, University of Arkansas for Medical Science
Additional Information
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C. Avoid repeated freeze / thaw cycles |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
